High level production of tyrosinase in recombinant Escherichia coli
نویسندگان
چکیده
منابع مشابه
High level production of tyrosinase in recombinant Escherichia coli
BACKGROUND Tyrosinase is a bifunctional enzyme that catalyzes both the hydroxylation of monophenols to o-diphenols (monophenolase activity) and the subsequent oxidation of the diphenols to o-quinones (diphenolase activity). Due to the potential applications of tyrosinase in biotechnology, in particular in biocatalysis and for biosensors, it is desirable to develop a suitable low-cost process fo...
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E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls th...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2013
ISSN: 1472-6750
DOI: 10.1186/1472-6750-13-18